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Santa Cruz Biotechnology itga6 antibody

<t>ITGA6</t> is the regulatory target of INHBA. ( A ) Differential expression genes (DEGs) heat map identified by RNA-seq. ( B ) The number of DEGs identified by RNA-seq. ( C ) Overlap analysis of RNA-seq and STAD-DEGs-up identified genes. ( D ) Expression of ITGA6 in GC paired tissue cohort from the GEPIA2 database. ( E ) Subcellular localization of ITGA6 from GeneCards. ( F , G ) The interaction between INHBA and ITGA6 was verified by Co-IF ( F ) and Co-IP ( G ) experiments. Scale bar, 50 μm. ( H ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA knockdown in NUGC-3 cells. ( I ) WB was used to detect ITGA6 protein expression after INHBA knockdown in NUGC-3 cells. ( J ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA overexpression in AGS cells. ( K ) WB was used to detect ITGA6 protein expression after INHBA overexpression in AGS cells. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001

Itga6 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/itga6+antibody/pmc12963699-154-10-14?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 173 article reviews

itga6 antibody - by Bioz Stars, 2026-07

93/100 stars

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1) Product Images from "INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6"

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

Journal: Oncology Research

doi: 10.32604/or.2025.070333

ITGA6 is the regulatory target of INHBA. ( A ) Differential expression genes (DEGs) heat map identified by RNA-seq. ( B ) The number of DEGs identified by RNA-seq. ( C ) Overlap analysis of RNA-seq and STAD-DEGs-up identified genes. ( D ) Expression of ITGA6 in GC paired tissue cohort from the GEPIA2 database. ( E ) Subcellular localization of ITGA6 from GeneCards. ( F , G ) The interaction between INHBA and ITGA6 was verified by Co-IF ( F ) and Co-IP ( G ) experiments. Scale bar, 50 μm. ( H ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA knockdown in NUGC-3 cells. ( I ) WB was used to detect ITGA6 protein expression after INHBA knockdown in NUGC-3 cells. ( J ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA overexpression in AGS cells. ( K ) WB was used to detect ITGA6 protein expression after INHBA overexpression in AGS cells. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001

Figure Legend Snippet: ITGA6 is the regulatory target of INHBA. ( A ) Differential expression genes (DEGs) heat map identified by RNA-seq. ( B ) The number of DEGs identified by RNA-seq. ( C ) Overlap analysis of RNA-seq and STAD-DEGs-up identified genes. ( D ) Expression of ITGA6 in GC paired tissue cohort from the GEPIA2 database. ( E ) Subcellular localization of ITGA6 from GeneCards. ( F , G ) The interaction between INHBA and ITGA6 was verified by Co-IF ( F ) and Co-IP ( G ) experiments. Scale bar, 50 μm. ( H ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA knockdown in NUGC-3 cells. ( I ) WB was used to detect ITGA6 protein expression after INHBA knockdown in NUGC-3 cells. ( J ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA overexpression in AGS cells. ( K ) WB was used to detect ITGA6 protein expression after INHBA overexpression in AGS cells. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001

Techniques Used: Quantitative Proteomics, RNA Sequencing, Expressing, Co-Immunoprecipitation Assay, Quantitative RT-PCR, Knockdown, Over Expression

Expression of ITGA6 and its interaction with INHBA in GC. ( A ) The basal expression of ITGA6 mRNA in GC cell lines and GES-1 was detected by qRT-PCR. ( B ) The basal expression of ITGA6 protein in cell lines was detected by WB. ( C ) The expression of ITGA6 protein in 30 pairs of GC paired tissues was detected by IHC. (magnification ×400). The red arrow indicates the ITGA6 expression area, which appears brown or yellow. ( D,E ) Four siRNA-ITGA6 sequences were transfected simultaneously into AGS cells. ( F,G ) The relative expression level of INHBA mRNA and protein was detected by qRT-PCR and WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( H,I ) The relative expression level of INHBA mRNA and protein were detected by qRT-PCR and WB after instantaneous co-transfection of ITGA6 siRNA and INHBA overexpression plasmid in AGS. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Figure Legend Snippet: Expression of ITGA6 and its interaction with INHBA in GC. ( A ) The basal expression of ITGA6 mRNA in GC cell lines and GES-1 was detected by qRT-PCR. ( B ) The basal expression of ITGA6 protein in cell lines was detected by WB. ( C ) The expression of ITGA6 protein in 30 pairs of GC paired tissues was detected by IHC. (magnification ×400). The red arrow indicates the ITGA6 expression area, which appears brown or yellow. ( D,E ) Four siRNA-ITGA6 sequences were transfected simultaneously into AGS cells. ( F,G ) The relative expression level of INHBA mRNA and protein was detected by qRT-PCR and WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( H,I ) The relative expression level of INHBA mRNA and protein were detected by qRT-PCR and WB after instantaneous co-transfection of ITGA6 siRNA and INHBA overexpression plasmid in AGS. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques Used: Expressing, Quantitative RT-PCR, Transfection, Cotransfection, Over Expression, Plasmid Preparation

Oncogenic function of INHBA depends on ITGA6 and the MAPK signaling pathway. ( A ) KEGG enrichment result of the differentially expressed gene sets from the GEPIA2 database. ( B ) The expression levels of MAPK signaling pathway-related proteins were detected by WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( C,E,G,I,J ) The effects of ITGA6 on INHBA in NUGC-3 cells were detected by CCK-8 assay ( C ), colony formation assay ( E ), wound healing assay ( G ), Scale bar, 200 μm, Transwell migration assay ( I ) and Transwell invasion assay ( J ). Scale bar, 100 μm. ( D,F,H,K,L ) The effects of ITGA6 on INHBA in AGS cells were detected by CCK-8 assay ( D ), colony formation assay ( F ), wound healing assay ( H ), Scale bar, 200 μm, Transwell migration assay ( K ) and Transwell invasion assay ( L ). Scale bar, 100 μm. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Figure Legend Snippet: Oncogenic function of INHBA depends on ITGA6 and the MAPK signaling pathway. ( A ) KEGG enrichment result of the differentially expressed gene sets from the GEPIA2 database. ( B ) The expression levels of MAPK signaling pathway-related proteins were detected by WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( C,E,G,I,J ) The effects of ITGA6 on INHBA in NUGC-3 cells were detected by CCK-8 assay ( C ), colony formation assay ( E ), wound healing assay ( G ), Scale bar, 200 μm, Transwell migration assay ( I ) and Transwell invasion assay ( J ). Scale bar, 100 μm. ( D,F,H,K,L ) The effects of ITGA6 on INHBA in AGS cells were detected by CCK-8 assay ( D ), colony formation assay ( F ), wound healing assay ( H ), Scale bar, 200 μm, Transwell migration assay ( K ) and Transwell invasion assay ( L ). Scale bar, 100 μm. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques Used: Expressing, Cotransfection, Over Expression, Plasmid Preparation, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay

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Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: ITGA6 is the regulatory target of INHBA. ( A ) Differential expression genes (DEGs) heat map identified by RNA-seq. ( B ) The number of DEGs identified by RNA-seq. ( C ) Overlap analysis of RNA-seq and STAD-DEGs-up identified genes. ( D ) Expression of ITGA6 in GC paired tissue cohort from the GEPIA2 database. ( E ) Subcellular localization of ITGA6 from GeneCards. ( F , G ) The interaction between INHBA and ITGA6 was verified by Co-IF ( F ) and Co-IP ( G ) experiments. Scale bar, 50 μm. ( H ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA knockdown in NUGC-3 cells. ( I ) WB was used to detect ITGA6 protein expression after INHBA knockdown in NUGC-3 cells. ( J ) qRT-PCR was used to detect ITGA6 mRNA expression after INHBA overexpression in AGS cells. ( K ) WB was used to detect ITGA6 protein expression after INHBA overexpression in AGS cells. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Primary INHBA antibody (diluted 1:50; Proteintech; Catalog number: 17524-1-AP) and ITGA6 antibody (diluted 1:50; Santa Cruz Biotechnology, Dallas, TX, USA; Catalog number: sc-374057) were added to the cells and incubated overnight at 4°C.

Techniques: Quantitative Proteomics, RNA Sequencing, Expressing, Co-Immunoprecipitation Assay, Quantitative RT-PCR, Knockdown, Over Expression

Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: Expression of ITGA6 and its interaction with INHBA in GC. ( A ) The basal expression of ITGA6 mRNA in GC cell lines and GES-1 was detected by qRT-PCR. ( B ) The basal expression of ITGA6 protein in cell lines was detected by WB. ( C ) The expression of ITGA6 protein in 30 pairs of GC paired tissues was detected by IHC. (magnification ×400). The red arrow indicates the ITGA6 expression area, which appears brown or yellow. ( D,E ) Four siRNA-ITGA6 sequences were transfected simultaneously into AGS cells. ( F,G ) The relative expression level of INHBA mRNA and protein was detected by qRT-PCR and WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( H,I ) The relative expression level of INHBA mRNA and protein were detected by qRT-PCR and WB after instantaneous co-transfection of ITGA6 siRNA and INHBA overexpression plasmid in AGS. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques: Expressing, Quantitative RT-PCR, Transfection, Cotransfection, Over Expression, Plasmid Preparation

Journal: Oncology Research

Article Title: INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6

doi: 10.32604/or.2025.070333

Figure Lengend Snippet: Oncogenic function of INHBA depends on ITGA6 and the MAPK signaling pathway. ( A ) KEGG enrichment result of the differentially expressed gene sets from the GEPIA2 database. ( B ) The expression levels of MAPK signaling pathway-related proteins were detected by WB after instantaneous co-transfection of INHBA siRNA and ITGA6 overexpression plasmid in NUGC-3. ( C,E,G,I,J ) The effects of ITGA6 on INHBA in NUGC-3 cells were detected by CCK-8 assay ( C ), colony formation assay ( E ), wound healing assay ( G ), Scale bar, 200 μm, Transwell migration assay ( I ) and Transwell invasion assay ( J ). Scale bar, 100 μm. ( D,F,H,K,L ) The effects of ITGA6 on INHBA in AGS cells were detected by CCK-8 assay ( D ), colony formation assay ( F ), wound healing assay ( H ), Scale bar, 200 μm, Transwell migration assay ( K ) and Transwell invasion assay ( L ). Scale bar, 100 μm. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques: Expressing, Cotransfection, Over Expression, Plasmid Preparation, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay

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